Coding

Part:BBa_K2587008:Design

Designed by: Susanne Vollmer   Group: iGEM18_Duesseldorf   (2018-09-18)


guaD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
    Illegal NotI site found at 2
    Illegal NotI site found at 1378
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal SpeI site found at 18
    Illegal SpeI site found at 1371
    Illegal AgeI site found at 840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 25
    Illegal BsaI.rc site found at 1364


Design Notes

Added Golden Gate overhangs, to make it compatible with the CIDAR Toolbox from Iverson et al in 2015.


Source

amplified with PCR from the E.coli DH5α genome.

References